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HA-Tag Monoclonal An...

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HA-Tag Monoclonal Antibody

　　恒远生物抗体平台现已拥有4万多种抗体(含单抗和多抗)，包括人类常用抗体，模式生物类相关抗体、小分子抗体、二抗、标签抗体及内参抗体等。并以每年4000种的速度在持续增长。

　　恒远生物拥有专业的质控团队，配备先进实验仪器，现已成功地建立了ChIP平台，因此我们的抗体不仅能提供Biotin、FITC、HRP等标记，大部分抗体都能进行ELISA、WB、IHC、IF、IP、ChIP等应用验证。

　　我们致力于为所有用户提供高亲和力、高特异性的优质抗体。

产品详情 相关产品

HA-Tag Monoclonal Antibody

 
  • 货号：
    HYK-200061
  • 规格：
     
  • 图片：

    • Western Blot
      Positive WB detected in: 3 different overexpression lysates with HA tagged
      All lanes: HA-Tag antibody at 1:1000
      Secondary
      Goat polyclonal to Mouse IgG at 1/10000 dilution
      Predicted band size: 35, 35, 48 kDa
      Observed band size: 35, 35, 48 kDa

    • Western Blot
      Positive WB detected in: HA-tagged fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng, 1.5625ng
      All lanes: HA-Tag antibody at 1:1000
      Secondary
      Goat polyclonal to Mouse IgG at 1/10000 dilution
      Predicted band size: 35 kDa
      Observed band size: 35 kDa

    • Western Blot
      Positive WB detected in: HA-tagged fusion protein
      All lanes: HA-Tag antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000
      Secondary
      Goat polyclonal to Mouse IgG at 1/10000 dilution
      Predicted band size: 35 kDa
      Observed band size: 35 kDa

    • Western Blot
      Positive WB detected in: 6 different recombinant proteins with HA tagged, Hela whole cell lysate, 3T3 whole cell lysate
      All lanes: HA-Tag antibody at 1:5000
      Secondary
      Goat polyclonal to Mouse IgG at 1/10000 dilution

    • Immunofluorescence staining of 293F cells with CSB-MA000141M0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

产品详情

  • 别名：
    HA-Tag
  • 宿主：
    Mouse
  • 反应种属：
    All
  • 免疫原：
    YPYDVPDYA synthetic peptide conjugate to KLH
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    IgG2b
  • 纯化方式：
    >95%, Protein A purified
  • 克隆号：
    18B11H6
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IF, IP, FC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:5000-1:160000
    IF 1:50-1:200
    IP 1µg-5µg
    FC 1:50-1:200
  • Protocols：
    ELISA Protocol
    Western Blotting (WB) Protocol
    Immunofluorescence (IF) Protocol
    Immunoprecipitation (IP) Protocol
    Flow cytometry (FC) Protocol
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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