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Myc tag Monoclonal Antibody

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产品详情相关产品

Myc tag Monoclonal Antibody

货号：

HYK-200067
规格：
图片：
WB: Mouse anti Myc-tagged fusion protein Monoclonal antibody at 1.6μg/ml
Lane 1: Recombinant Myc-tagged fusion protein at 50ng
Lane 2: Recombinant Myc-tagged fusion protein at 25ng
Lane 3: Recombinant Myc-tagged fusion protein at 12.25ng
Lane 4: Recombinant Myc-tagged fusion protein at 6.25ng
Lane 5: Recombinant Myc-tagged fusion protein at 3.125ng
Lane 6: Recombinant Myc-tagged fusion protein at 1.5625ng
Secondary
Goat polyclonal to Mouse IgG at 1/50000 dilution
Predicted band size: 50 kd
Observed band size: 50 kd

Immunofluorescence staining of transfected HEK293 cells with CSB-MA000041M0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

产品详情

产品描述：

Myc tag monoclonal antibody CSB-MA000041M0m was produced in the mouse immunized by using the EQKLISEEDL (Myc) synthetic peptide conjugate to KLH as the immunogen. The target peptide Myc is a popular short peptide tag (EQKLISEEDL) derived from the c-myc gene product and recognized by numerous commercial antibodies as a tag. This tag can be added to the C- or N-terminus by recombinant DNA technology and can be used for affinity chromatography and for isolating protein complexes with multiple subunits.
This Myc tag monoclonal antibody was tested in the ELISA, WB, IF and IP. The non-conjugated IgG1 got purified by protein G and reached up to 95% in purity. It can be used for detection and expression of Myc tag fusion expression proteins, intracellular localization, and purification, qualitative or quantitative detection of Myc fusion-expressed proteins and the like. It doesn’t have species restricted.
别名：

bHLHe39, c Myc, MRTL, MYC, Myc proto oncogene protein, MYC tag, Proto oncogene c Myc, Transcription factor p64
宿主：

Mouse
反应种属：

N/A
免疫原：

EQKLISEEDL (Myc) synthetic peptide conjugate to KLH
标记方式：

Non-conjugated
克隆类型：

Monoclonal
抗体亚型：

IgG1
纯化方式：

>95%, Protein G purified
克隆号：

5F92F9
浓度：

It differs from different batches. Please contact us to confirm it.
保存缓冲液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
产品提供形式：

Liquid
应用范围：

ELISA, WB, IF, IP

推荐稀释比：

Application	Recommended Dilution
WB	1:500-1:5000
IF	1:100-1:300
IP	1:1000-1:1500

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
储存条件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
货期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.