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Flag Tag Monoclonal Antibody

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产品详情相关产品

Flag Tag Monoclonal Antibody

货号：

HYK-200074
规格：
图片：
Western Blot
Positive WB detected in: 10ng Flag Tag fusion protein Flag Tag antibody at 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa

Western Blot
Positive WB detected in: Flag Tag fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.625ng, 1.5625ng, 0.78ng
All lanes: Flag Tag antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa

Western Blot
Positive WB detected in: Flag Tag fusion protein 1, 2, 3, 4 at 50ng
All lanes: Flag Tag antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 59.6, 36, 35, 42 kDa
Observed band size: 59.6, 36, 35, 42 kDa

Western Blot
Positive WB detected in: Flag Tag fusion protein at 10ng
All lanes: Company A, Company B, Company C, Company D, CSB-MA000021M0m antibody at 1:50000, 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000, 1:3200000, 1:6400000
Secondary
Goat polyclonal to Mouse IgG at 1/50000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa

Western Blot
Positive WB detected in: Flag Tag fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng, 1.5625ng, 0.78ng, 0.39ng
All lanes: Company A, Company B, Company C, Company D, CSB-MA000021M0m antibody at 1:1000
Secondary
Goat polyclonal to Mouse IgG at 1/50000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa

产品详情

产品描述：

Flag Tag monoclonal antibody CSB-MA000021M0m was produced in the mouse immunized by using the DYKDDDDK(Flag) synthetic peptide conjugate to KLH as the immunogen. The Flag tag, a popular short peptide tag (DYKDDDDK), can be fused to the C- terminus or N-terminus of the protein. It is used in recombinant DNA technology and can be used for affinity chromatography and can also be used with other affinity tags.
This Flag Tag Monoclonal Antibody was tested in the WB, IF, IP and ELISA applications. The non-conjugated IgG2a got purified by protein G and reached up to 95% in purity. Flag antibodies can be used to detect and express the expression of Flag-tagged fusion proteins, intracellular localization, and to purify, qualitatively or quantitatively detect Flag fusion-expressed proteins. It doesn’t have species restricted.
别名：

DDK tag, DYKDDDDK, Flag, Flag Tag
宿主：

Mouse
反应种属：

N/A
免疫原：

DYKDDDDK(Flag) synthetic peptide conjugate to KLH
标记方式：

Non-conjugated
克隆类型：

Monoclonal
抗体亚型：

IgG2a
纯化方式：

>95%, Protein G purified
克隆号：

4G5C8
浓度：

It differs from different batches. Please contact us to confirm it.
保存缓冲液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
产品提供形式：

Liquid
应用范围：

ELISA, WB, IF, IP

推荐稀释比：

Application	Recommended Dilution
WB	1:1000-1:10000
IF	1:100-1:300
IP	0.5ul-2ul

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
储存条件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
货期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.