欢迎选择我们，上海恒远生物科技有限公司！免费服务热线：400-0899-208 防伪查询

语言：Eng

您当前的位置：: 首页>; 产品中心>; 抗体>; 多克隆抗体>; GFAP Antibody

多克隆抗体

GFAP Antibody

　　恒远生物抗体平台现已拥有4万多种抗体(含单抗和多抗)，包括人类常用抗体，模式生物类相关抗体、小分子抗体、二抗、标签抗体及内参抗体等。并以每年4000种的速度在持续增长。

　　恒远生物拥有专业的质控团队，配备先进实验仪器，现已成功地建立了ChIP平台，因此我们的抗体不仅能提供Biotin、FITC、HRP等标记，大部分抗体都能进行ELISA、WB、IHC、IF、IP、ChIP等应用验证。

　　我们致力于为所有用户提供高亲和力、高特异性的优质抗体，欢迎来电咨询。

产品详情相关产品

GFAP Antibody

货号：

HYK-200116
规格：
图片：
Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate
All lanes: GFAP antibody at 3.9µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 50, 51 kDa
Observed band size: 50 kDa

IHC image of CSB-PA009369LA01HU diluted at 1:200 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA009369LA01HU diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of A549 cells with CSB-PA009369LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

产品详情

产品名称：

Rabbit anti-Homo sapiens (Human) GFAP Polyclonal antibody
Uniprot No.：

P14136
基因名：

GFAP
别名：

GFAP antibody; GFAP Epsilon antibody; GFAP_HUMAN antibody; GFAPdelta antibody; GFAPepsilon antibody; Glial fibrillary acidic protein antibody; Intermediate filament protein antibody
宿主：

Rabbit
反应种属：

Human
免疫原：

Recombinant Human Glial fibrillary acidic protein (292-432AA)
免疫原种属：

Homo sapiens (Human)
标记方式：

Non-conjugated
克隆类型：

Polyclonal
抗体亚型：

IgG
纯化方式：

>95%, Protein G purified
浓度：

It differs from different batches. Please contact us to confirm it.
保存缓冲液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
产品提供形式：

Liquid
应用范围：

ELISA, WB, IHC, IF

推荐稀释比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
储存条件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
货期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.