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CD81 Antibody

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产品详情相关产品

CD81 Antibody

货号：

HYK-200086
规格：
图片：
Western Blot
Positive WB detected in:K562 whole cell lysate
All lanes:CD81 antibody at 1.25μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 26 KDa
Observed band size: 26 KDa

IHC image of CSB-RA004960A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-RA004960A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Overlay histogram showing Jurkat cells stained with CSB-RA004960A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

产品详情

Uniprot No.：

P60033
基因名：

CD81
别名：

26 kDa cell surface protein TAPA 1 antibody; 26 kDa cell surface protein TAPA-1 antibody; 26 kDa cell surface protein TAPA1 antibody; CD 81 antibody; CD81 antibody; CD81 antigen (target of antiproliferative antibody 1) antibody; CD81 antigen antibody; CD81 molecule antibody; CD81_HUMAN antibody; CVID6 antibody; S5.7 antibody; TAPA 1 antibody; TAPA1 antibody; Target of the antiproliferative antibody 1 antibody; Tetraspanin 28 antibody; Tetraspanin-28 antibody; Tetraspanin28 antibody; Tspan 28 antibody; Tspan-28 antibody; Tspan28 antibody
反应种属：

Human, Mouse, Rat
免疫原：

A synthesized peptide
免疫原种属：

Homo sapiens (Human)
标记方式：

Non-conjugated
克隆类型：

Monoclonal
抗体亚型：

Rabbit IgG
纯化方式：

Affinity-chromatography
浓度：

It differs from different batches. Please contact us to confirm it.
保存缓冲液：

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
产品提供形式：

Liquid
应用范围：

ELISA, WB, IHC, FC

推荐稀释比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:500

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow cytometry (FC) Protocol
储存条件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
货期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.