Recombinant Human Macrophage migration inhibitory factor(MIF)

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活性蛋白

Recombinant Human Macrophage migration inhibitory factor(MIF): 恒远生物蛋白表达平台建立了从原核到真核四大重组表达系统,并建立无细胞表达系统,拥有基于昆虫病毒外膜蛋白展示的蛋白表达技术、膜蛋白表达技术等一系列蛋白表达及纯化技术,有能力制备各种高难度蛋白。

产品详情相关产品

Recombinant Human Macrophage migration inhibitory factor(MIF)

货号：

HYB-100163
规格：
图片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

产品详情

纯度：

Greater than 87% as determined by SDS-PAGE.
内毒素：

Less than 1.0 EU/ug as determined by LAL method.
生物活性：

①Measured by its binding ability in a functional ELISA. Immobilized MIF at 2 μg/ml can bind Anti- MIF Rabbit Monoclonal Antibody（CSB-RA146975A0HU）, the EC50 is 49.61-69.45 ng/ml.
基因名：

MIF

别名：

(MIF)(Glycosylation-inhibiting factor)(GIF)(L-dopachrome isomerase)(L-dopachrome tautomerase)(Phenylpyruvate tautomerase)
分子结构：
种属：

Homo sapiens (Human)
蛋白长度：

Full Length of Mature Protein
来源：

Mammalian cell
分子量：

41.3 kDa
表达区域：

2-115aa
氨基酸序列

PMFIVNTNVPRASVPDGFLSELTQQLAQATGKPPQYIAVHVVPDQLMAFGGSSEPCALCSLHSIGKIGGAQNRSYSKLLCGLLAERLRISPDRVYINYYDMNAANVGWNNSTFA
蛋白标签：

C-terminal hFc-tagged
产品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
缓冲液：

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
复溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
储存条件：

Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保质期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
货期：

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
产品描述：

Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
Datasheet & COA：

Please contact us to get it.